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Bacteria commonly exist in multicellular, surface-attached communities called biofilms. Biofilms are central to ecology, medicine, and industry. TheVibrio choleraepathogen forms biofilms from single founder cells that, via cell division, mature into three-dimensional structures with distinct, yet reproducible, regional architectures. To define mechanisms underlying biofilm developmental transitions, we establish a single-molecule fluorescence in situ hybridization (smFISH) approach that enables accurate quantitation of spatiotemporal gene-expression patterns in biofilms at cell-scale resolution. smFISH analyses ofV. choleraebiofilm regulatory and structural genes demonstrate that, as biofilms mature, overall matrix gene expression decreases, and simultaneously, a pattern emerges in which matrix gene expression becomes largely confined to peripheral biofilm cells. Both quorum sensing and c-di-GMP-signaling are required to generate the proper temporal pattern of matrix gene expression. Quorum sensing signaling is uniform across the biofilm, and thus, c-di-GMP-signaling alone sets the regional matrix gene expression pattern. The smFISH strategy provides insight into mechanisms conferring particular fates to individual biofilm cells. 

Abstract The time-variable emission from the accretion flow of Sgr A*, the supermassive black hole at the Galactic center, has long been examined in the radio-to-millimeter, near-infrared (NIR), and X-ray regimes of the electromagnetic spectrum. However, until now, sensitivity and angular resolution have been insufficient in the crucial mid-infrared (MIR) regime. The MIRI instrument on JWST has changed that, and we report the first MIR detection of Sgr A*. The detection was during a flare that lasted about 40 minutes, a duration similar to NIR and X-ray flares, and the source's spectral index steepened as the flare ended. The steepening suggests that synchrotron cooling is an important process for Sgr A*'s variability and implies magnetic fields strengths ~ 40–70 G in the emission zone. Observations at 1.3 mm with the Submillimeter Array revealed a counterpart flare lagging the MIR flare by ≈10 minutes. The observations can be self-consistently explained as synchrotron radiation from a single population of gradually cooling high-energy electrons accelerated through (a combination of) magnetic reconnection and/or magnetized turbulence. 

This study evaluates the beneficial effects of discharging nanosecond pulse transient plasma (NPTP) in a coaxial electrostatic precipitator for capturing nanoscale soot particles (∼50 nm) produced by an ethylene flame. 

Optical coatings play a vital role in sensing technologies. The development of new coatings that exhibit minimal optical losses requires a detailed understanding of the development of defects within them. Current methods of defect characterization involve direct microscope imaging or x-ray diffraction studies in the case of crystallites. In this paper, we demonstrate the characterization of coating defects using light scattering, which can yield information about their size, location, and index of refraction. The method requires measuring the scattered power of each individual defect as a function of angle and comparing the data with theoretical models. Finally, we argue that this method can be used for the determination of the defect location within a multi-layer stack. 

Abstract Most bacteria in the biosphere are predicted to be polylysogens harbouring multiple prophages^1–5. In studied systems, prophage induction from lysogeny to lysis is near-universally driven by DNA-damaging agents⁶. Thus, how co-residing prophages compete for cell resources if they respond to an identical trigger is unknown. Here we discover regulatory modules that control prophage induction independently of the DNA-damage cue. The modules bear little resemblance at the sequence level but share a regulatory logic by having a transcription factor that activates the expression of a neighbouring gene that encodes a small protein. The small protein inactivates the master repressor of lysis, which leads to induction. Polylysogens that harbour two prophages exposed to DNA damage release mixed populations of phages. Single-cell analyses reveal that this blend is a consequence of discrete subsets of cells producing one, the other or both phages. By contrast, induction through the DNA-damage-independent module results in cells producing only the phage sensitive to that specific cue. Thus, in the polylysogens tested, the stimulus used to induce lysis determines phage productivity. Considering the lack of potent DNA-damaging agents in natural habitats, additional phage-encoded sensory pathways to lysis likely have fundamental roles in phage–host biology and inter-prophage competition.